Introduction to Ultra Clean Workbench
The ultra-clean bench is a facility that provides a sterile, dust-free operating environment for laboratory work to protect the experiment from the external environment while providing some degree of protection to the external environment from pollution and protecting the operator. Compared with the simple aseptic cover, the clean bench has the advantages of allowing the operator to move freely, easily reaching anywhere in the operating area, and being more comprehensive. At present, the ultra-clean workbench used in China has imported products, such as BIOGARD B40-112, which is an ultra-clean workbench that meets the U.S. Class A safety standard. It is a biological organism that tends to have low and medium risks. Products recommended for academic trials.
- Principle: The clean environment of the ultra-clean workbench is formed in a specific space, and the clean air (filtered air) flows in a set direction. Divided by the direction of airflow, the existing ultra-clean worktable can be divided into vertical type, inside-out type, and side-type. the vertical type is superior. The clean air provided by the air supply filter plate descends through the operation area at a specific speed and is separated in the middle of the operation area. It is sucked away by the front air suction hole and the rear suction window. the same volume of air is replenished from the room air through the operating port. This air never enters the operating area; it just forms an air barrier. Many domestic ultra-clean workbenches only have air supply filter plates. The filtered air enters the operating area to form a certain positive pressure. The air is discharged from the exhaust holes and operation ports into the ambient air. This airflow mode affects the surrounding environment and operation. No one has a protective effect.
- The ultra-clean workbench where the debugger is located undergoes rigorous testing before leaving the factory to ensure normal use. But this does not mean that the manufacturer’s test can replace the operator’s necessary inspection and debug before use. Because the environment of ultra-clean workbenches is different, only by proper inspection and debugging can the function of the equipment be maximized? A good environment should be selected for the machine before commissioning. It is best to place it in a sterile room with air disinfection facilities. If conditions are not available, the machine should be placed in a cleaner room with less movement. Adjust the height of each foot to ensure the stability and level of the operation surface. A special circuit should be used for the power supply of the ultra-clean bench to avoid the change of airflow rate caused by circuit overload. Compared with the simple aseptic cover, the ultra-clean bench has the freedom to allow the operator to move freely and easily reach anywhere in the operating area. Ultraviolet germicidal lamps and fluorescent lighting lamps are the standard configurations of the ultra-clean bench. The blower provides the power for airflow. It is clear at a glance whether these parts are working properly. The most difficult and important thing is to check the air filter plate and its tightness, which is directly related to the normal use of the machine. The simplest method is the nutrient agar plate method. Newly purchased and long-used ultra-clean workbenches should be fumigated in addition to being irradiated with UV lamps. Then, when the machine is in the working state, place an open nutrient agar plate on each of the four corners and the center of the operating area. After two hours, cover and incubate in a 37 ° C incubator for 24 hours to calculate the number of colonies. The average number of colonies per plate must be less than 0.5.
- Use the ultra-clean workbench before applying UV light for 30-40 minutes, and check that the various openable doors and windows around the operating area are in the working position. The operation is best performed in the center of the operating area, which is a safer area by design. You should have a preliminary understanding of the experimental materials before performing the operation and understand the performance and safety level of the equipment you use. Strict implementation of laboratory safety procedures. The use of specific pathogens in any ultra-clean bench must be evaluated for safety. If the experimental materials cause environmental pollution to the surrounding environment, it should be avoided in the model without an exhaust filter plate, because operating inflowing air is no different from detoxification. Any advanced equipment does not guarantee the success of the experiment. The use of ultra-clean benches in animal quarantine laboratories is for sterility and avoiding cross-contamination. Therefore, skilled operation and clear sterility essentials are essential.
- Maintenance: The ultra-clean workbench is a relatively precise electrical equipment. It is imperative to perform regular maintenance and maintenance on it. First of all, keep the room dry and clean. Humid air will not only rust the manufacturing materials but also affect the normal operation of electrical circuits. Humid air will also help the growth of bacteria and mold. A clean environment also extends the life of the filter plate. In addition, regular cleaning of equipment is an important part of normal use. Cleaning should include routine cleaning and regular handling before and after use. During a fumigation, all gaps should be completely sealed. If the operation port is equipped with a removable baffle cover, a clean bench can be sealed with a plastic film. The filter plates and ultra-violet germicidal lamps of ultra-clean workbenches have a specified service life and should be replaced on schedule.